The aim of this milestone is to integrate different Payload modules to perform live-cell imaging, which is the main (and probably most critical) in-orbit operation of the Payload. Another description for this would be to run one sub-experiment:

• end-to-end (e.g. 48-72 hours)
• with the maximum number of developed modules (DM, EQM-like DM)
• with the maximum representativeness in its operational sequence

The collected data would be cell growth and fluorescence intensity (gene expression) time courses (brightfield and GFP images).

The first version of this platform (which will hopefully be further developed to become a Payload-FlatSat) can include the following:

• Biomolecular System: cells (in SD-His) or spores (in SD-His-Leu), inserted via the inlets or spotted
• Imaging System: AcubeSAT imaging system or Dino-Lite setup
• Microfluidic System: biodisplay single-layer (e.g. control), or biodisplay and MITOMI updated versions
• Fluidic System: "Earth-based" pneumatic system
• Temperature Control System: temperature control chamber or heaters with closed-loop control
• Electronics-PCBs: DM (or random) boards in the lab. Laptops also work for some components (e.g. microscope)