[TESTING] - Imaging | Fluorescent agent

Description

Test the imaging system's ability (with the excitation LED module) to capture fluorescence levels of a fluorescent agent, perfused in a microfluidic chip. In other words, create a standard/calibration curve.

Notes:

• The agent is fluorescein sodium or any other dye close to GFP's excitation/emission spectrum. You can also try to use its pH dependent fluorescence properties, as explained here.
• Check out this protocol on a fluorescence standard curve with fluorescein in Calibration 3: Fluorescence standard curve - Fluorescein Protocol
• To simulate different levels, you can perform serial dilutions of the agent in water or PBS
• Single-layer chips can be used
• Most likely, you won't saturate the signal so your curve, well, will be a linear line.