[TESTING] - Imaging | Cells (fluorescence, chip, spotted)

Description

Test the imaging system's ability (with the excitation LED module) to capture fluorescence levels of fluorescent cells. The cells are spotted on a glass slide and then integrated in a microfluidic chip.

Notes:

• The cells can be any of the S. cerevisiae cells or spores that we have in the lab. If cells are spotted, the experiment will need to run after a maximum of 1 hour.
• To simulate different levels, 2 ways are doable:
  • Transforming a GFP plasmid with an inducible promoter into a non-fluorescent S. cerevisiae strain and while running the chip using a medium supplemented with different levels of inducer. However, with the current chip architecture, the experiments will need to run in different chips and thus comparisons won't be ideal.
  • Transforming a GFP plasmid library with constitutive promoters (or RBSs) of varying strength into non-fluorescent S. cerevisiae strains. These cells can then be spotted in different chambers and thus different levels can be tested on the same chip.
• Single-layer flow chips can be used (no need for double-layer chips since this is part of the old design).
• A relevant requirement is ASAT-MIS-150: