[TESTING] - Imaging | Fluorescent cells (plate)

Description

Test the imaging system's ability (with the excitation LED module) to capture fluorescence levels of fluorescent cells. The cells are can be in liquid culture in a multi-well plate (we could also have them on agar plate, but liquid culture is easier).

Notes:

1. The cells can be E. coli, transformed with a GFP expressing plasmid "library" and each construct expresses GFP at different level. For example, from the iGEM collection:

Sample	Plasmid	Coordinate	Backbone	Promoter	Strength*
Negative Control	BBa_J428100	Kit Plate 1 Well 12M	pSB1C3
Positive Control	BBa_I20270	Kit Plate 1 Well 1A	pSB1C3	J23151**
Test Device 1	BBa_J364000	Kit Plate 1 Well 1C	pSB1C3	J23101	1791
Test Device 2	BBa_J364001	Kit Plate 1 Well 1E	pSB1C3	J23106	1185
Test Device 3	BBa_J364002	Kit Plate 1 Well 1G	pSB1C3	J23117	162
Test Device 4	BBa_J364007	Kit Plate 1 Well 1I	pSB1C3	J23100	2547
Test Device 5	BBa_J364008	Kit Plate 1 Well 1K	pSB1C3	J23104	1831
Test Device 6	BBa_J364009	Kit Plate 1 Well 1M	pSB1C3	J23116	396

*Measured in Variant RFP (au) in the iGEM Registry wiki page.

**Different RBS (B0032 instead of B0034).

***These refer to the Spring 2022 Distribution (check the Interlab protocol).

2. The cells can also be S. cerevisiae cells that we have in the lab. Specifically, one of the housekeeping strains, e.g. RPL8A / YHL033C or RNR3 / YIL066C.

• Another way to simulate different levels is by preparing a type of cell co-culture. In this case, you can use a non-fluorescent strain and a fluorescent strain and mix them in different ratios. Assuming the cells grow at the same rate (identical/similar genetic background etc), you can easily get different fluorescent levels per cell population ratio.
• A relevant requirement is ASAT-MIS-150: