Busco fails on simple genome
Hello, I try to run Busco with autolineage on this test data MAG. it is a MAG from a simulated metagenome and should be a Streptococus sp.
Busco autolineage fails with the following log:
2022-10-13 17:21:06 INFO: Results: C:97.6%[S:97.6%,D:0.0%],F:0.8%,M:1.6%,n:124
2022-10-13 17:21:06 INFO: bacteria_odb10 selected
2022-10-13 17:21:06 INFO: ***** Searching tree for chosen lineage to find best taxonomic match *****
2022-10-13 17:21:07 INFO: Extract markers...
2022-10-13 17:21:08 INFO: Place the markers on the reference tree...
2022-10-13 17:21:08 INFO: Running 1 job(s) on sepp, starting at 10/13/2022 17:21:08
2022-10-13 17:21:50 INFO: [sepp] 1 of 1 task(s) completed
2022-10-13 17:21:51 ERROR: Placements failed. Try to rerun increasing the memory or select a lineage manually.
I provided 60GB RAM. And I don't understand why a genome with 97% completeness fails the tree placing. For comparison, checkM predicts 99.7% completeness at the genus level.