tblastn makeblastdb error - not creating blast database
When I run Busco I get this error where it says that makeblastdb is complete but it does not create a blast database for the next step. Do you know why it's not working? For reference, I am using blast version 2.2.31 and busco version 4.0.6. Thanks!
INFO: Creating BLAST database with input file INFO: Running 1 job(s) on makeblastdb INFO: [makeblastdb] 1 of 1 task(s) completed ERROR: DB /Volumes/KUDLES/Echinoderm Genomes/P miniata genome 3.0/unpurged_busco/blast_db/pmin.ctg.fa not found for tblastn job ERROR: BUSCO analysis failed !
In the makeblastdb error log I have this error:
BLAST options error: Please provide a database name using -out
and here is the more detailed error log
INFO:busco.BuscoTools Creating BLAST database with input file INFO:busco.Toolset Running 1 job(s) on makeblastdb DEBUG:busco.Toolset cmd call: /Users/carolynku/anaconda2/envs/bsucoenv/bin/makeblastdb -in pmin_igv/pmin.ctg.fa -dbtype nucl -out /Volumes/KUDLES/Echinoderm Genomes/P miniata genome 3.0/unpurged_busco/blast_db/pmin.ctg.fa INFO:busco.Toolset [makeblastdb] 1 of 1 task(s) completed ERROR:busco.run_BUSCO DB /Volumes/KUDLES/Echinoderm Genomes/P miniata genome 3.0/unpurged_busco/blast_db/pmin.ctg.fa not found for tblastn job DEBUG:busco.run_BUSCO DB /Volumes/KUDLES/Echinoderm Genomes/P miniata genome 3.0/unpurged_busco/blast_db/pmin.ctg.fa not found for tblastn job Traceback (most recent call last): File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/run_BUSCO.py", line 196, in run_BUSCO busco_run.run_analysis() File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/BuscoRunner.py", line 70, in run_analysis raise se File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/BuscoRunner.py", line 41, in run_analysis self.analysis.run_analysis() File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/GenomeAnalysis.py", line 416, in run_analysis coords = self._run_tblastn() File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/Analysis.py", line 106, in _run_tblastn incomplete_buscos) File "/Users/carolynku/anaconda2/envs/bsucoenv/lib/python3.6/site-packages/busco/BuscoTools.py", line 867, in __init__ raise SystemExit("DB {} not found for tblastn job".format(self.blast_db)) SystemExit: DB /Volumes/KUDLES/Echinoderm Genomes/P miniata genome 3.0/unpurged_busco/blast_db/pmin.ctg.fa not found for tblastn job ERROR:busco.run_BUSCO BUSCO analysis failed ! ERROR:busco.run_BUSCO Check the logs, read the user guide, and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues