Commit 366b498b authored by harm's avatar harm
Browse files


parent 0609a756
......@@ -21,6 +21,9 @@ tar -xzf Cas13design.tar.gz
cd Cas13design
cat Install.txt
## Example
......@@ -42,6 +45,9 @@ ls -ltr ./data/*fasta
### Predict guide RNA scores
Next, simply execute RfxCas13d_GuideScoring.R by providing 3 required input arguments:
......@@ -61,9 +67,10 @@ If you run the script the first time, R package installation may take several mi
Once Cas13design is fully installed, the run time scales with the fasta input length.
Our software extracts all information needed from the target input sequence, including base probabilities, RNA-RNA hybridization energies, RNA target site accessibility or guide RNA folding.
The minimum length supplied must be at least 30 nucleotides. The total run time for the provided ~1000nt test.fa example is about 2 min.
### Output
The output may be 3-fold and named after the fasta header information:
Markdown is supported
0% or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment