Commit acc4125e authored by Joel Collins's avatar Joel Collins

Added placeholder for reflection illumination assembly

parent 6c7e3b69
......@@ -24,17 +24,18 @@ The basic version of the microscope uses a webcam lens instead of a microscope o
* 3 [feet](./parts/printed/feet.md): ``feet.stl`` or ``feet_tall.stl`` (contains all 3)
* 3 [large gears](./parts/printed/gears.md): ``gears.stl`` (contains all 3)
* illumination:
- [vertical dovetail](./parts/printed/illumination_dovetail.md): ``illumination_dovetail.stl``
- [condenser arm](./parts/printed/condenser.md): ``condenser.stl``
* [vertical dovetail](./parts/printed/illumination_dovetail.md): ``illumination_dovetail.stl``
* [condenser arm](./parts/printed/condenser.md): ``condenser.stl``
* 2 [sample clips](./parts/printed/sample_clips.md): ``sample_clips.stl`` (contains both)
* ``camera_platform_picamera_2_LS65.stl``
* ``lens_spacer_picamera_2_pilens_LS65.stl``
* platform-style optics module (two parts, best with webcam lenses):
- [camera platform](./parts/printed/camera_platform.md): ``camera_platform_<camera>_<stage size><height>.stl``
- [lens spacer](./parts/printed/lens_spacer.md): ``lens_spacer_<camera>_<lens>_<stage_size><height>.stl``
* ``microscope_stand.stl`` (or ``back_foot.stl``)
* [camera platform](./parts/printed/camera_platform.md): ``camera_platform_<camera>_<stage size><height>.stl``
* [lens spacer](./parts/printed/lens_spacer.md): ``lens_spacer_<camera>_<lens>_<stage_size><height>.stl``
* [microscope stand](./parts/printed/microscope_stand.md) or back foot
* ``microscope_stand_no_pi_.stl`` or ``microscope_stand-30.stl`` (holds Raspberry Pi) or ``back_foot.stl``
This will need the nuts, bolts, etc. described below, plus a camera module (for example a Raspberry Pi camera), and an option Raspberry Pi. No other optical bits are required, except an LED for illumination.
This will need the nuts, bolts, etc. described below, plus a Raspberry Pi computer (any model will do) and Raspberry Pi camera module. No other optical bits are required, except an LED for illumination. If you want to mount the Raspberry Pi underneath the microscope (our preferred option), print ``microscope_stand.stl`` instead. If your Raspberry Pi is in a case already, you should print ``back_foot.stl`` so the microscope sits flat on a table. Newer camera modules include a white circular tool for unscrewing the lens. If you don't have one, you will need to print the lens gripper (marked as optional).
If you want to mount a Raspberry Pi underneath the microscope (our preferred option), print ``microscope_stand.stl`` instead. If your Raspberry Pi is in a case already, you should print ``back_foot.stl`` so the microscope sits flat on a table. Newer Raspberry Pi camera modules include a white circular tool for unscrewing the lens. If you don't have one, you will need to print the lens gripper (marked as optional).
### High resolution microscope
......@@ -44,37 +45,42 @@ The version of the microscope used for scientific or medical research generally
* [band and nut insertion tools](./parts/printed_tools/actuator_assembly_tools.md) ``actuator_assembly_tools.stl``
* [tool to insert](./parts/printed_tools/lens_tool.md) the 13mm diameter condenser lens and/or tube lens: ``lens_tool.stl``
* [optional] ``picamera_2_lens_gripper.stl`` (only needed if your camera didn't come with a tool to remove the lens)
**Components:**
* [body of the microscope](./parts/printed/main_body.md) (beamsplitter-compatible): ``main_body_LS65-M-BS.stl`` or ``main_body_LS65-M-BS_brim.stl``.
* ``picamera_2_gripper.stl``
* [optional] ``picamera_2_lens_gripper.stl`` (only needed if your camera didn't come with a tool to remove the lens)
* [body of the microscope](./parts/printed/main_body.md): ``main_body_LS65-M.stl``.
* 3 [feet](./parts/printed/feet.md): ``feet.stl`` or ``feet_tall.stl`` (contains all 3)
* 3 [large gears](./parts/printed/gears.md): ``gears.stl`` (contains all 3)
* illumination:
- [vertical dovetail](./parts/printed/illumination_dovetail.md): ``illumination_dovetail.stl``
- [condenser arm](./parts/printed/condenser.md): ``condenser.stl``
* [vertical dovetail](./parts/printed/illumination_dovetail.md): ``illumination_dovetail.stl``
* [condenser arm](./parts/printed/condenser.md): ``condenser.stl``
* 2 [sample clips](./parts/printed/sample_clips.md): ``sample_clips.stl`` (contains both)
* [optics module](./parts/printed/optics_module_casing.md): ``optics_picamera_2_rms_f50d13.stl`` or ``optics_picamera_2_rms_f50d13_beamsplitter.stl``
* [base to hold a Raspberry Pi](./parts/printed/microscope_stand.md): ``microscope_stand.stl``
* [optics module](./parts/printed/optics_module_casing.md):
* ``optics_picamera_2_rms_f50d13.stl`` (transmission illumination)
* ``optics_picamera_2_rms_f50d13_beamsplitter.stl`` (reflection illumination)
* [microscope stand](./parts/printed/microscope_stand.md): ``microscope_stand-30.stl`` or ``microscope_stand-30-BS.stl`` (supports reflection illumination)
* [riser for the sample](./parts/printed/sample_riser.md): ``sample_riser_LS10.stl`` (assuming you have a microscope objective with a 45mm parfocal distance)
**For motorised operation you will also need**
**For motorised operation you will also need:**
* 3 [small gears](./parts/printed/small_gears.md) for motors: ``small_gears.stl`` (contains all 3)
* [base to hold the motor driver](./parts/printed/motor_driver_case.md) (fits under the base that holds the Pi): ``motor_driver_case.stl``
**For reflection illumination you will also need**
**For reflection illumination you will also need:**
* [filter cube](./parts/printed/fl_cube.md): ``fl_cube.stl``
* [reflection illuminator](./parts/printed/reflection_illuminator.md): ``reflection_illuminator.stl``
**Additionally, you will need:**
Additionally, you will need:
* an RMS threaded, finite-conjugates [objective lens](parts/optics/objective.md). These can be obtained from e.g. AliExpress. Depending on whether it is 35mm or 45mm from the "shoulder" of the lens to the sample, you may or may not need the sample riser. We almost always use 45mm "plan" corrected lenses, which do require the riser.
* a 12.7mm diameter, 50mm focal length [achromatic lens](parts/optics/tube_lens.md), e.g. ThorLabs ac127-050-a or generic equivalent.
* a 13mm diameter, 5mm focal length PMMA plano convex lens for the [condenser lens](parts/optics/condenser_lens.md) (sold as LED lenses in bulk)
* [motors](./parts/stepper_motors.md) and [motor driver electronics](6_motor_controllers.md)
## Print settings
I usually print with a layer size of 0.24mm on my Ormerod, which takes 10 hours for the main body. "low" quality on an Ultimaker 2 (0.15mm layers) produces similar results in about 10 hours. Our Prusa i3 Mk3 takes a similar time using 0.2mm layers, or 0.3mm layers if we want to go even faster (8 hours or less).
> **Warning:** The microscope is designed to print without support material. If you use support material it will require a lot of cleaning up, and you may well damage the parts.
......
# Assemble the reflection illumination
The microscope can illuminate the sample from below, allowing imaging of reflected, instead of transmitted, light. This section takes you through the assembly of the reflection illumination arm.
# Requirements
## Parts
* 1 [5mm LED](./parts/electronics/white_led.md) ideally wired up to a 2-way female header
* 1 [condenser lens](./parts/optics/condenser_lens.md)
* 1 [filter cube](./parts/printed/fl_cube.md)
* Requires 1.5mm thick optical filters (eg. 50/50 splitter, or dichroic filters) to insert
* 1 [reflection illuminator](./parts/printed/reflection_illuminator.md)
## Tools
* [Condenser lens insertion tool](./parts/printed_tools/lens_tool.md)
# Assembly Instructions
......@@ -5,7 +5,9 @@
- [Optics module](#)
- [Basic optics module](./2a_basic_optics_module.md)
- [High resolution optics module](./2b_high_resolution_optics_module.md)
- [Illumination](./3_illumination.md)
- [Illumination](#)
- [Transmission illumination](./3_illumination.md)
- [Reflection illumination](./3b_illumination_reflection.md)
- [Sample clips and wiring](./4_clips_and_wiring.md)
- [Motors](./5_motors.md)
- [Motor controller boards](./6_motor_controllers.md)
\ No newline at end of file
......@@ -10,7 +10,7 @@ Assembly is broken up into several steps:
* [Printing parts](./0_printing.md)
* [Assembling the actuators](./1_actuator_assembly.md) deals with the mechanism for moving the stage.
* The optics module comes next, either the [basic optics module](./2a_basic_optics_module.md) or the [high resolution optics module](./2b_high_resolution_optics_module.md) depending on your needs.
* [Illumination](./3_illumination.md) covers attaching the condenser.
* [Illumination](./3_illumination.md) covers attaching the condenser for transmission illumination. [Reflection illumination](./3b_illumination_reflection.md) covers optional reflection illumination.
* [Sample clips and wiring](./4_clips_and_wiring.md) completes the microscope.
* [Motors](./5_motors.md) can be added for automatic control.
* [Motor controller boards](./6_motor_controllers.md) are required for driving motors.
......
# Microscope feet
The feet serve mainly to anchor the Viton bands that tension the actuators, but if you are not using a stand (we reccommend that you do) they also serve to support the microscope. You need two tilted feet and one untilted foot, as provided in ``feet.stl``. In some circumstances you may need to use taller feet, as provided in ``feet_tall.stl`` but this is now deprecated, in favour of using a microscope stand (which means you don't need the tall feet).
The feet serve mainly to anchor the Viton bands that tension the actuators, but if you are not using a stand (we reccommend that you do) they also serve to support the microscope.
You need two tilted feet and one untilted foot, as provided in ``feet.stl``.
In some circumstances you may need to use taller feet, as provided in ``feet_tall.stl`` but this is now deprecated, in favour of using a microscope stand (which means you don't need the tall feet).
# Reflection/fluorescence cube
The filter cube allows for reflection illumination, by inserting a 50/50 beamsplitter filter into the 45 degree slot. It also enables fluorescence microscope, by instead inserting a dichroic filter in the 45 degree slot, and a long-pass filter in the bottom slot.
The cube is provided in a single file ``fl_cube.stl``.
Note, this must be used in conjunction with the [reflection illuminator](./reflection_illuminator.md).
......@@ -2,6 +2,7 @@
The stand will hold the microscope flat on a table, and can also house a Raspberry Pi computer to control the microscope. It can optionally stack on top of the [motor driver case](./motor_driver_case.md) if you need to mount a motor controller.
## STL Files
There are two versions:
* ``microscope_stand.stl`` holds a Raspberry Pi.
There are three versions:
* ``microscope_stand_30.stl`` holds a Raspberry Pi.
* ``microscope_stand_30-BS.stl`` holds a Raspberry Pi and supports the [reflection illuminator](./reflection_illuminator).
* ``microscope_stand_no_pi`` is less tall, and doesn't hold the Pi.
# Reflection illuminator
The reflection illuminator allows sample iullumination in a reflection geometry, and must be used with a [filter cube](./fl_cube.md). Three parts are required, and are contained in a single file ``reflection_illuminator.stl``.
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