Fewer BUSCOs with BUSCOv4?
I previously ran BUSCO using the embryophta lineage with a large genome assembly (~20GB):
611 Complete BUSCOs (C) 452 Complete and single-copy BUSCOs (S) 159 Complete and duplicated BUSCOs (D) 107 Fragmented BUSCOs (F) 722 Missing BUSCOs (M) 1440 Total BUSCO groups searched
I tried running BUSCO on the same assembly but with BUSCO v4.0.2 and odb10:
550 Complete BUSCOs (C) 423 Complete and single-copy BUSCOs (S) 127 Complete and duplicated BUSCOs (D) 238 Fragmented BUSCOs (F) 826 Missing BUSCOs (M) 1614 Total BUSCO groups searched
It seems that fewer complete BUSCOs are found? Is there any explanation for why this could happen, or any parameters I could tweak to help fix this?