Metaeuk issue running BUSCO on transcriptome mode
Dear BUSCO team,
I have been trying to run BUSCO in transcriptome mode using CONDA (BUSCO version 5.8.3 and 5.3.2) and a container version (docker://ezlabgva/busco:v5.8.2_cv1) on an HPC server, but I always encounter the same issue. My testing sample/command is:
busco -i BisSp1.Trinity.fasta -m transcriptome -c 8 \
-f -l coleoptera_odb12 \
--out_path /My_PATH/TEST \
-o BisSp_BUSCO
BUSCO error is:
2025-05-12 15:32:12 INFO: ***** Start a BUSCO v5.8.2 analysis, current time: 05/12/2025 15:32:12 *****
2025-05-12 15:32:12 INFO: Configuring BUSCO with local environment
2025-05-12 15:32:12 INFO: Running transcriptome mode
2025-05-12 15:32:12 INFO: 'Force' option selected; overwriting previous results directory
2025-05-12 15:32:12 INFO: Downloading information on latest versions of BUSCO data...
2025-05-12 15:32:14 INFO: Input file is BisSp1.Trinity.fasta
2025-05-12 15:32:18 INFO: Running BUSCO using lineage dataset coleoptera_odb12 (eukaryota, 2025-04-11)
2025-05-12 15:32:18 INFO: Running 1 job(s) on metaeuk, starting at 05/12/2025 15:32:18
2025-05-12 15:32:20 INFO: [metaeuk] 1 of 1 task(s) completed
2025-05-12 15:32:20 ERROR: Metaeuk did not recognize any genes matching the dataset coleoptera_odb12 in the input file. If this is unexpected, check your input file and your installation of Metaeuk
2025-05-12 15:32:20 ERROR: BUSCO analysis failed!
2025-05-12 15:32:20 ERROR: Check the logs, read the user guide (https://busco.ezlab.org/busco_userguide.html), and check the BUSCO issue board on https://gitlab.com/ezlab/busco/issues
And looking inside the folders the metaeuk error is:
Failed to execute /My_PATH/TEST/BisSp1_BUSCO/run_coleoptera_odb12/metaeuk_output/tmp/1098942550272613958/easypredict.sh with error 13.
I looked into issue #706 (closed) and did not find the flag noexec in the /etc/fstab
Then... how can I solve this?
Thanks in advance for any help. kind regards, Niko
Edited by NiKo LiChi