Large difference of score between odb10 and odb12
Hello,
For the same genome assembly, I run BUSCO version 5.8.3 using ascomycota_odb10 and ascomycota_odb12 and the difference is huge as you can see below:
# BUSCO version is: 5.8.3
# The lineage dataset is: ascomycota_odb10 (Creation date: 2024-01-08, number of genomes: 365, number of BUSCOs: 1706)
# Summarized benchmarking in BUSCO notation for file FCM10-ONT-flye.fasta
# BUSCO was run in mode: euk_genome_min
# Gene predictor used: miniprot
***** Results: *****
C:92.6%[S:90.1%,D:2.5%],F:0.5%,M:7.0%,n:1706,E:0.4%
1579 Complete BUSCOs (C) (of which 6 contain internal stop codons)
1537 Complete and single-copy BUSCOs (S)
42 Complete and duplicated BUSCOs (D)
8 Fragmented BUSCOs (F)
119 Missing BUSCOs (M)
1706 Total BUSCO groups searched
Assembly Statistics:
31 Number of scaffolds
31 Number of contigs
12422343 Total length
0.000% Percent gaps
812 KB Scaffold N50
812 KB Contigs N50
# BUSCO version is: 5.8.3
# The lineage dataset is: ascomycota_odb12 (Creation date: 2025-02-25, number of genomes: 105, number of BUSCOs: 2826)
# Summarized benchmarking in BUSCO notation for file FCM10-ONT-flye.fasta
# BUSCO was run in mode: euk_genome_min
# Gene predictor used: miniprot
***** Results: *****
C:49.4%[S:46.5%,D:2.9%],F:5.2%,M:45.4%,n:2826,E:0.3%
1395 Complete BUSCOs (C) (of which 4 contain internal stop codons)
1313 Complete and single-copy BUSCOs (S)
82 Complete and duplicated BUSCOs (D)
148 Fragmented BUSCOs (F)
1283 Missing BUSCOs (M)
2826 Total BUSCO groups searched
Assembly Statistics:
31 Number of scaffolds
31 Number of contigs
12422343 Total length
0.000% Percent gaps
812 KB Scaffold N50
812 KB Contigs N50
Is there an explanation for this difference? Thank you.