Trying this out; does not run monocle3 code

I pulled the docker from your site and started it It leaves me at a bash prompt

from https://cole-trapnell-lab.github.io/monocle3/monocle3_docs/#installing-monocle

I can then launch R

BiocManager::install(c('BiocGenerics', 'DelayedArray', 'DelayedMatrixStats', 'limma', 'S4Vectors', 'SingleCellExperiment', 'SummarizedExperiment'))

neither of these next lines worked install.packages("reticulate") reticulate::py_install("louvain")

library(monocle) worked but I was expecting to use 'monocle3'

the following three lines worked

Load the data

expression_matrix <- readRDS(url("http://staff.washington.edu/hpliner/data/cao_l2_expression.rds")) cell_metadata <- readRDS(url("http://staff.washington.edu/hpliner/data/cao_l2_colData.rds")) gene_annotation <- readRDS(url("http://staff.washington.edu/hpliner/data/cao_l2_rowData.rds"))

but this next line failed

cds <- new_cell_data_set(expression_matrix,cell_metadata = cell_metadata,gene_metadata = gene_annotation) Error in new_cell_data_set(expression_matrix, cell_metadata = cell_metadata, : could not find function "new_cell_data_set"

and also

cds <- newCellDataSet(expression_matrix,cell_metadata = cell_metadata,gene_metadata = gene_annotation) Error in newCellDataSet(expression_matrix, cell_metadata = cell_metadata, : unused arguments (cell_metadata = cell_metadata, gene_metadata = gene_annotation)

Question why can't the software recognize "new_cell_data_set"

Thanks

Starr