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**Command line example**:
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```bash
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samtools view -F 256 --output-fmt BAM <in.sam>
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samtools view -F 256 --bam --output <out.bam> <in.sam>
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```
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**Arguments**:
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- _-F \<flag\>_: only include reads with none of the flags present
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- _--output-fmt \<SAM/BAM/CRAM\>_: convert file to SAM, BAM or CRAM format
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- _--bam_: output in the BAM format
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- _--output \<out.bam\>_: output file name
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#### Samtools faidx
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