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**Command line example**:
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```bash
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samtools view -F 0x100 --output-fmt BAM -o <out.bam> <in.sam>
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samtools view -F 0x100 -b -o <out.bam> <in.sam>
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```
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**Arguments**:
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- _-F \<flag\>_: only include reads with none of the flags present
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- _--output-fmt \<SAM/BAM/CRAM\>_: output in the format SAM, BAM or CRAM
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- _-b_: output in the format BAM
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- _-o \<out.bam\>_: output file name
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#### Samtools faidx
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