- _--thread_ \<numberOfThreads\>_: number of threads to use for the processing
- _--thread<numberOfThreads>_: number of threads to use for the processing
With this simple usage command line, by default, fastp will quality filtering reads with a mean phred quality below 15 and allow 40% of bases to be unqualified. It will also filter based on a minimum length requirement, and filter reads which have a complexity below 30%. To finish, fastp will trim adapters based on which are detected.
- _--nthreads \<numberOfThreads\>_: number of threads to use for the processing (default value: 8)
- _--genomedb \<genomeName\>_: name of the genome (index files prefix)
- _--nthreads <numberOfThreads>_: number of threads to use for the processing (default value: 8)
- _--genomedb <genomeName>_: name of the genome (index files prefix)
- _--dir path/to/somewhere/_: directory where index files will be written
Then, paired-end RNA-Seq data are aligned against the database created as described above. Input files must be in FASTQ or FASTA format. The FASTQ input may include quality scores, which will then be included in SAM output:
- _-F \<flag\>_: only include reads with none of the flags present
- _-b_: output in the format BAM
- _-o \<out.bam\>_: output file name
- _-F <flag>_: only include reads with none of the flags present
- _-b_: output in BAM format
- _-o <out.bam>_: output file name
#### Samtools faidx
...
...
@@ -225,8 +225,8 @@ PsiCLASS is an accurate and efficient transcript assembler which simultaneously
**Arguments**:
- _-b \<listOfBamFiles\>_: list of BAM files separated by comma
- _-o \<output.gtf\>_: path to output file
- _-b <listOfBamFiles>_: list of BAM files separated by comma
- _-o <output.gtf>_: path to output file
*Note*: If there is a "-" in one file path, PsiCLASS will return a segmentation fault ! So it's important to avoid them when naming directories or files.